Bupivacaine is chemically (RS)-1-butyl-N-(2, 6-dimethylphenyl) piperidine-2-carboxamide is used for the local anaesthetic. As Bupivacaine hydrochloride is official in IP, BP, and USP, no official analytical method is there for estimation of this drug. Literature survey reveals few analytical methods like liquid chromatography, stability indicating HPLC, colorimetric and biological assay are reported; there was no mention of a method based on spectrophotometric estimation. The present work describes a validated zero and first order derivative Spectrophotometric method measurement for estimation of Bupivacaine. The present work describes a validated Zero and first order derivative Spectrophotometric method for estimation of Bupivacaine in bulk and in parentral formulation. The solvent used was Sodium Hydroxide dilution. An absorbance maximum in zero order is and first order derivative spectra was found at Absorbance minima at 272 nm, the drug followed a linear relationship in the range of 10-35μg/ml while the correlation coefficient was found 0.999. The recovery was 99.89% and the coefficient of variance for intraday and interday was found to be less than 5%, LOD and LOQ for this method was found 10μg/ml and 35μg/ml respectively. The proposed UV- Visible spectrophotometric method is a simple, specific, rapid, accurate, economic and found suitable for day to day analysis of Bupivacaine in bulk and its formulation.
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